Request QuoteCatalog Number: xP372121BOXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP372121BOXYeast1mgQuote
EP372121BOXE. coli1mgQuote
BP372121BOXBaculovirus200ugQuote
MP372121BOXMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
UniProt IDA1A280
Gene NamemiaB; Locus:BAD_1032
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-480
Expression Tag6xHis
Purity>90%
AA SequenceMMTEAERASKLAEPAANDTPTRGKGVFYVHTLGCQMNVHDSERIAGVLEADGYVPATEEQ YLNHDIDLIVMNTCAVRENAAERMYGTIGLWAELKRERPNLQIAVGGCMAQLDREKIAKK APWVDAVFGTKNIGSLPQLLDQARIEGHAQVKVQEELNYFPSQLPTDRASKVSSWVAISV GCNNTCTFCIVPTTRGKEHDRRPGDILAEIRQCVDEGAKEVTLLGQNVNSFGYGIGDRFA FSKLLRACGEIDGLERVRFTSPHPAAFTDDVIAAMAETPNVMHQLHFPLQSGSDRILRAM RRSYRSAKFLDILRKIREAMPDAQISTDIIVGFPGETEEDFQETLRVVEEARFASAFTFI YSPRPGTPAAEMEQVPHDVVQDRFERLVALQERITEENLKTFEGRDVEVMVTGASGKKDA ATHRVTGREKTGVLVHVGVPEGEPMPQVGDFVTATVTHAGRHNLIADPNPEAGQTYAVRH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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