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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP874289DJAYeast1mgQuote
EP874289DJAE. coli1mgQuote
BP874289DJABaculovirus200ugQuote
MP874289DJAMammalian Cell200ugQuote

Protein Information

SpeciesDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
UniProt IDQ9RV79
Gene NamemiaB; Locus:DR_1150
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-505
Expression Tag6xHis
Purity>90%
AA SequenceMGQKAKAQAPTTHPRPHTLSSQVAPALPRRPRHAAPESLRQARGEVMKAHLITYGCQMNE YDTHLVQSQLVSFGADIVESPDEADFVLVNTCAVRGKPVDKVRSLLGDLRKQKAQRSLVV GMMGCLAQLEEGQQIARKFEVDVLLGPGSLLDIGAALESNERFWGLQFKDELHDHIPPPP SGKLQAHLTIMRGCDHHCTYCIVPTTRGPQVSRHPDDILRELDMQLAAGVREVTLLGQNV NAYGVDQGAKLKGYPSFADLLRMVGASGIERVKFTTSHPMNFTEDVAAAIGETPAICEFV HLPVQSGSDRVLRRMAREYNREKYLTHIAQIKKHIPDVVLATDIIVGFPGETEEDFQDTL SLYDEVGYDSAYMFIYSPRPGTPSYKHFQDLPRELKTERLQRLIARQKDWSARKNAQKVG TVQQVLLRGDAHDAGFLEGHTRGNHPTVVPKAIGADGAGVYQVRIDHATPHMMYGHILGP DGQPLPEQPRFNPEAAAVGGALPML
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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