Request QuoteCatalog Number: xP894873TNJSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP894873TNJYeast1mgQuote
EP894873TNJE. coli1mgQuote
BP894873TNJBaculovirus200ugQuote
MP894873TNJMammalian Cell200ugQuote

Protein Information

SpeciesThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
UniProt IDQ9WZC1
Gene NamemiaB; Locus:TM_0653
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-443
Expression Tag6xHis
Purity>90%
AA SequenceMRFYIKTFGCQMNENDSEAMAGLLVKEGFTPASSPEEADVVIINTCAVRRKSEEKAYSEL GQVLKLKKKKKIVVGVAGCVAEKEREKFLEKGADFVLGTRAVPRVTEAVKKALEGEKVAL FEDHLDEYTHELPRIRTSRHHAWVTIIHGCDRFCTYCIVPYTRGRERSRPMADILEEVKK LAEQGYREVTFLGQNVDAYGKDLKDGSSLAKLLEEASKIEGIERIWFLTSYPTDFSDELI EVIAKNPKVAKSVHLPVQSGSNRILKLMNRRYTKEEYLALLEKIRSKVPEVAISSDIIVG FPTETEEDFMETVDLVEKAQFERLNLAIYSPREGTVAWKYYKDDVPYEEKVRRMQFLMNL QKRINRKLNERYRGKTVRIIVEAQAKNGLFYGRDIRNKIIAFEGEDWMIGRFADVKVEKI TAGPLYGKVVWVEKTPSPVSSSE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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