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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP821872RKUYeast1mgQuote
EP821872RKUE. coli1mgQuote
BP821872RKUBaculovirus200ugQuote
MP821872RKUMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
UniProt IDQ92SI7
Gene NamemiaB; Locus:R00405; ORFs:SMc01115
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-472
Expression Tag6xHis
Purity>90%
AA SequenceMTQETALLSTSPEGGDLNVPARKVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEDAD FVLLNTCHIREKAAEKVYSELGRLRELKKAKALEGREMLIGVAGCVAQAEGDEILRRVPA VDLVIGPQTYHRLPDALKRARAGQRIVETEYAIEDKFVHLPAPDKAKTRARGVTAFLTVQ EGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGEGPHGR EWSLGDLLRRLGEIDGLARLRYTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRIL KAMNRRHTAAEYLALVERIRAAQPDLALSGDFIVGFPGETDQDFEDTLRLVEEVNYAQAF SFKYSTRPGTPGAELKEQVPEDVKAKRLEILQALLVKQQRGFAEACVGREIDLLLEKPGR MPGQLVGRSPWLQPVNVDAKASQIGDIIRVRITKAGPNSLFAEMIGESDARS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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