Request QuoteCatalog Number: xP851069XAYSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP851069XAYYeast1mgQuote
EP851069XAYE. coli1mgQuote
BP851069XAYBaculovirus200ugQuote
MP851069XAYMammalian Cell200ugQuote

Protein Information

SpeciesXanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
UniProt IDQ8P8B5
Gene NamemiaB; Locus:XCC2328
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-485
Expression Tag6xHis
Purity>90%
AA SequenceMPGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVL AASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVAS QEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRADG PSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAY RGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANY LHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKT MKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQARLARLQAHINAHAAGISQRMV GSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQL DDSAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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