Request QuoteCatalog Number: xP814834TDQSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP814834TDQYeast1mgQuote
EP814834TDQE. coli1mgQuote
BP814834TDQBaculovirus200ugQuote
MP814834TDQMammalian Cell200ugQuote

Protein Information

SpeciesThermosynechococcus elongatus (strain BP-1)
UniProt IDQ8DJB2
Gene NamemiaB; Locus:tlr1315
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMPRRYYITTFGCQMNKADSERMAGILEAMGLELAAEPDEADVLLYNTCTIRDNAEQKLYS YLGRQAKRKHQDPNLTLIVAGCVAQQEGEQLLRRVPEVDLVMGPQYANRLGELLEQVWNG SQVVATEPLQIVEDITKPRRDSTVTAWVNVIYGCNERCTYCVVPGVRGQEQSRRPEAIRA EIEELAAQGYKEVTLLGQNIDAYGRDLPGITPEGRRQHTFTDLLYYIHDVAGIERIRFAT SHPRYFTERLIRACAELPKVCKHFHIPFQSGDNEILKAMARGYTRERYLHIIETIRRYMP DAAISADAIVGFPGETEEQFQRTLDLVAAVGFDQLNTAAYSPRPNTPAATWENQVPEAIK EDRLQRLNHLVAKIAGDRSQRYLGREEVVLVEGVNPKDAQQVYGRTDGNRLTYLPGDIET LRGQLVRVRITEARAFSLSGVPLAASSLVC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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