Request QuoteCatalog Number: xP756156RDRSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP756156RDRYeast1mgQuote
EP756156RDRE. coli1mgQuote
BP756156RDRBaculovirus200ugQuote
MP756156RDRMammalian Cell200ugQuote

Protein Information

SpeciesRhodopirellula baltica (strain SH1)
UniProt IDQ7ULM9
Gene NamemiaB; Locus:RB9401
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-479
Expression Tag6xHis
Purity>90%
AA SequenceMISMTKTVYIKTVGCQMNVLDSEMVIADLKRHGYTVVDTPGEADLLLYNTCSIREQAEEK TYSALGKLKETKARHPEKTIGVMGCMAQKDQETIFRRAPFVDMVVGPGQLHAIPDMLTKV TSGEGRQMAVSLGRKDGKQTVVARSHETFDPLRDPTMRPTPFQAYLRIQIGCDKFCTYCV VPNTRGPEQGRSPEEIVSEARVLAEQGALEITLLGQTVNSYRHRGPDGETDMAGLLERLH DIDGLKRIKFVTNYPKDMTARLLETIRDLPKVSPYLHVPAQSGSDAVLKRMKRGYTIADY MEMFERIETVLPEASVSSDFIVGFCGETDEDFQKSVKLIERCRFKNSFIFQYSVREGTKA AANLIDDVPREVKAARNNELLAVQDRISKEDNQKLIGDTVEVLVEGPSKKADKSDLDAPI VQMTGRTICDRIVVFDGNRRQAGQLMDIQIDDVSSHTLIGRVKTVEVVSLGMPGLAPSS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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