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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP745960SSLYeast1mgQuote
EP745960SSLE. coli1mgQuote
BP745960SSLBaculovirus200ugQuote
MP745960SSLMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain WH8102)
UniProt IDQ7U5R0
Gene NamemiaB; Locus:SYNW1640
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-460
Expression Tag6xHis
Purity>90%
AA SequenceMPAANAQSRGSYWITTFGCQMNKADSERMAGILESMGYRAANAELEADLVLYNTCTIRDN AEQKVYSYLGRQAQRKRLDPNLTLIVAGCVAQQEGESLLRRVPELDLVMGPQHANRLEVL LNRVDSGQQVVATEDHHILEDITTARRDSSICGWVNVIYGCNERCTYCVVPSVRGKEQSR LPEAIRLEMEGLAAQGYKEITLLGQNIDAYGRDLPGITPEGRRQHTLTDLLHQVHDVSGI KRIRFATSHPRYFTERLIDACADLPKLCEHFHIPFQSGDNDVLRAMARGYTVERYRRIID RIRERMPDASLSADVIVAFPGETDLQYQRTLDLIEEIGFDQVNTAAYSPRPNTPAATWDN QLPEEVKVIRLQTINALVERCARERNARYAGRTEEVLAEGINPKDPSQLMGRTRTNRLTF FQAAGPDGHQHNPGDLVNVRIDAVRSFSLSGTPLPCVEQR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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