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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP749640HSYYeast1mgQuote
EP749640HSYE. coli1mgQuote
BP749640HSYBaculovirus200ugQuote
MP749640HSYMammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus ducreyi (strain 35000HP / ATCC 700724)
UniProt IDQ7VP74
Gene NamemiaB; Locus:HD_0226
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMAKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADILLLNTCSIREKAQEKVFS QLGRWKNWKKDKPDLIIGVGGCVASQEGEHIRERAPFVDIVFGPQTLHRLPEMINQIRSG DRAVVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRLRYTTSHP IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLRAVRPNIQ ISSDFIVGFPGETAEDFEQTMKIIEQVNFDMSFSFIYSARPGTPASDLPDDVSENEKKDR LAHLQQRINHQAMQFSRLMLGTEQRILVEGPSKKDIMELTGRTETNRVVNFIGTPNMIGK FVDIKITDVYSNSLRGEVIRTEDQMGLRIVESASSVIARTRKEDDLGVGKYAVNL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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