Request QuoteCatalog Number: xP766810HCOSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP766810HCOYeast1mgQuote
EP766810HCOE. coli1mgQuote
BP766810HCOBaculovirus200ugQuote
MP766810HCOMammalian Cell200ugQuote

Protein Information

SpeciesHelicobacter hepaticus (strain ATCC 51449 / 3B1)
UniProt IDQ7VGP6
Gene NamemiaB; Locus:HH_1274
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-462
Expression Tag6xHis
Purity>90%
AA SequenceMKLFIQTLGCAMNERDSAHMIAELRDKKHYTLTNDIKQADLILINTCSVREKPEKKLFSE IGAFAKEKKAGAKIGVCGCTASHLGEEIIKKAPSVDFVLGARNVSKITQVLERPKAVEVD IDYDDSTYVFASSQGMGIKAHLNISIGCDKKCSYCIVPFTRGKEISVPKDLLISEAKKCV ASGAKELLLLGQNVNNYGVRFSHSHPKTNFTQLLRALSEIDGLYRIRFTSPHPLHMDDEF LEEFASNPVIAKGIHIPLQSGSSQILKMMRRGYDKQWYLNRIAKLKSLVPNVGIGTDIIV GFPTESEQDFEDTMEVLSLVEFDTLYSFVYSPRPHTSAFEYDKSMLVSPEVAKERLARLQ NLHKEILSKKAQLEIGRIHNVLIENHYNGEGQCWSEGRSSSNKLIKILDKKCEIGSIVKV EITHNEGGGLMGRFINELSLSEALASKEYFQNPIYIQEGALC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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