Request QuoteCatalog Number: xP772660TIWSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP772660TIWYeast1mgQuote
EP772660TIWE. coli1mgQuote
BP772660TIWBaculovirus200ugQuote
MP772660TIWMammalian Cell200ugQuote

Protein Information

SpeciesTropheryma whipplei (strain Twist) (Whipple's bacillus)
UniProt IDQ83FT9
Gene NamemiaB; Locus:TWT_610
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-470
Expression Tag6xHis
Purity>90%
AA SequenceMTYTVRTYGCQMNVHDSERIAGVLEDEGYVKSGSDDADVVVLNTCAVRENADNRFYGNLG QLLQKKNNGRIRQIAVGGCLAQKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKTA EIEIKDFLETFPSSLPVRRESNYSAWVSISVGCNNTCTFCIVPSLRGKERDRRPGDILAE ISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAAFTS DVIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAITTDI IVGFPGETEEDFQDTLNLVAEVRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYERLL DLQNRIALEENRKLIGKEVELLVTVGGRKDSMLDNRYTGRTPCGRLVHFSCLHQLRPGDF ATVKIIYAAPYHLIGDNALTVRRTPAGDVWIQKNDTRSAQLVSLGMPRIR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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