Request QuoteCatalog Number: xP745460VCQSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP745460VCQYeast1mgQuote
EP745460VCQE. coli1mgQuote
BP745460VCQBaculovirus200ugQuote
MP745460VCQMammalian Cell200ugQuote

Protein Information

SpeciesVibrio vulnificus (strain YJ016)
UniProt IDQ7MN00
Gene NamemiaB; Locus:VV0917
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEEPEEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKKPGVVIGVGGCVATQEGDHIRERAPFVDVIFGPQTLHRLPEMIKQSQS EDAPVMDISFPEIEKFDSLPEPRAEGATAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD VLYEIAQLAEQGVREVNLLGQNVNAYRGPMHDGEICSFAELLRLVASIDGIDRIRFTTSH PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTGIEYKSIIRKLRKARPDI QISSDFIVGFPGETDKDFQDTMKLIKDVDFDMSFSFIFSPRPGTPAADYPCDLSEETKKE RLYELQQQINAQAMRYSRLMLGTEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIG QFVDVKITDVFANSLRGEIVRTEKDMDLRSVISPTQMMAKTRREDELGVATFTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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