Request QuoteCatalog Number: xP745686CKASize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP745686CKAYeast1mgQuote
EP745686CKAE. coli1mgQuote
BP745686CKABaculovirus200ugQuote
MP745686CKAMammalian Cell200ugQuote

Protein Information

SpeciesChromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
UniProt IDQ7NQI8
Gene NamemiaB; Locus:CV_4149
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMKKVYIKTFGCQMNEYDSDKMADVLGSAEGMVKTDNPEEADVILFNTCSVREKAQEKVFS DLGRIRPLKEANPDLIIGVGGCVASQEGDAIVKRAPFVDVVFGPQTLHRLPDLIESRKQS GRSQVDISFPEIEKFDHIPPAKVDGGAAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV LTEIAGLAAQGVKEITLLGQNVNAYRGLMSDGEIADFALLLEYVHEVPGVERIRFTTSHP REFSQRIIDCYAKLPKLVSHLHLPVQSGSDRVLMAMKRGYTGLEYKSIIRKLRAIRPDLC LSSDFIIGFPGETEADFEQTLKLVRDCEFDFSFVFIYSPRPGTPAANLPDDTPHAEKVRR LEALNEVIEAKGYAINQSMVGTVQRVLVENVSKKDATMLAARTANNRVVNFAGHPRLLGR MIEVKITAAFPHSLAGEALTSESA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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