Request QuoteCatalog Number: xP749837BTYSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP749837BTYYeast1mgQuote
EP749837BTYE. coli1mgQuote
BP749837BTYBaculovirus200ugQuote
MP749837BTYMammalian Cell200ugQuote

Protein Information

SpeciesBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
UniProt IDQ7WMN3
Gene NamemiaB; Locus:BB1357
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMQETTVKRDGASPSDAGTPATTAQGLGKLYIRTFGCQMNEYDSDKMADVLRADQGLELTD NPEDADVILFNTCSVREKAQEKVFSDLGRVQHLKKQNPNLVIGVGGCVASQEGEAIVKRA PYVDVVFGPQTLHRLPDLIKRRRAQGVSQVDISFPEIEKFDALPPPRVDGATAFVSIMEG CSKYCSFCVVPYTRGEEVSRPFDDVLLEVADLADQGVKEVTLLGQNVNAYRGAMGDSGEI ADFAMLLEYVHEIPGIERIRYTTSHPKEMTQRMVDAYARLPKLVSFLHLPVQAGSDRVLA AMKRGYTALEFKSVVRRLRAARPSLTLSSDFIVGFPGETEEDFQKTMKLIEDVGFDTSFS FVYSRRPGTPAADLHDDTPQDVKLRRLQQLQALINQQAAAIAQGMIGTRQRVLVEGPSRR DPNELMGRTENNRIVNFPGVPRLIGHMVDVVVTHAHTNSLRGRVAGIERDTSGAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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