Request QuoteCatalog Number: xP749788BIKSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP749788BIKYeast1mgQuote
EP749788BIKE. coli1mgQuote
BP749788BIKBaculovirus200ugQuote
MP749788BIKMammalian Cell200ugQuote

Protein Information

SpeciesBordetella parapertussis
UniProt IDQ7WB66
Gene NamemiaB; Locus:BPP1141
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMQETTVKRDGASPSDAGTPATTAQGLGKLYIRTFGCQMNEYDSDKMADVLRADQGLELTD NPEDADVILFNTCSVREKAQEKVFSDLGRVQHLKKQNPNLVIGVGGCVASQEGEAIVKRA PYVDVVFGPQTLHRLPDLIKRRRAQGVSQVDISFPEIEKFDALPPPRVDGATAFVSIMEG CSKYCSFCVVPYTRGEEVSRPFDDVLLEIADLADQGVKEVTLLGQNVNAYRGAMGDSGEI ADFAMLLEYVHEIPGIERIRYTTSHPKEMTQRMVDAYARLPKLVSFLHLPVQAGSDRVLA AMKRGYTALEFKSVVRRLRAARPSLTLSSDFIVGFPGETEEDFQKTMKLIEDVGFDTSFS FVYSRRPGTPAADLHDDTPQDVKLRRLQQLQALINQQAAAIAQGMIGTRQRVLVEGPSRR DPNELMGRTENNRIVNFPGVPRLIGHMVDVVVTHAHTNSLRGRVAGIERDTSGAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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