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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP735429Yeast1mgQuote
EP735429E. coli1mgQuote
BP735429Baculovirus200ugQuote
MP735429Mammalian Cell200ugQuote

Protein Information

SpeciesAnaplasma marginale (strain St. Maries)
UniProt IDQ5PB69
Gene NamemiaB; Locus:AM401
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-455
Expression Tag6xHis
Purity>90%
AA SequenceMTKGLYIESYGCQMNVYDSLMVEDILRPLGFAAVQRPEDADIIMVNTCHVREKAAEKLYS ALGRMRMLRKEGALIVVAGCVAQAEGEAVFERAPFVDVVVGPQSIHTLPELIMKATRDAK QMNVEFPAISKFDVISTDLLVSRGVSAFVSVQEGCDKFCTFCVVPYTRGPEYSRSVEDVL AEVRKLADGGTKEVVLLGQNVNAYHGTYKGNEWDLGRLIRKVAEVEGIERIRYTTSHPRD MHSSLYDAHKHEPKLLPCVHLPVQSGSDSVLRKMNRKHSVQEYMDIIDTLKEARSDIALS SDFIVGFPGETEEDFEATMDLVRHVGFATSYSFKYSPRPGTPGAEYTNQVPEEEKSSRLH RLQHLLLTQQRLFTKSMIGKTVSVLVCGTEGSRSCSNEVFGKSEHMQPVYISVDGDPAGY RNKIFSVKVLEEGARSSLKGIICTDTISLPPPHSM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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