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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP692172Yeast1mgQuote
EP692172E. coli1mgQuote
BP692172Baculovirus200ugQuote
MP692172Mammalian Cell200ugQuote

Protein Information

SpeciesCampylobacter jejuni (strain RM1221)
UniProt IDQ5HW09
Gene NamemiaB; Locus:CJE0508
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-433
Expression Tag6xHis
Purity>90%
AA SequenceMSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKL FSEVGGFEKVKKEGAKIGVCGCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFM GVDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVPHTRGDEISIPFNIIYKEAQ KAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKEWYLNRALKLRELCPNVSISTD IIVAFPGESEKDFEETMDVLEKVRFEQIFSFKYSKRPLTKAATMPNQIDEETASRRLSTL QNRHSEILDEIVKKQENKTFKVLFEELRAGNSIAGRTDNNFLVQVEGSEELLGQFKEVKI TNAKRMVLYGEIV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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