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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP716622Yeast1mgQuote
EP716622E. coli1mgQuote
BP716622Baculovirus200ugQuote
MP716622Mammalian Cell200ugQuote

Protein Information

SpeciesBacillus clausii (strain KSM-K16)
UniProt IDQ5WFY0
Gene NamemiaB; Locus:ABC2190
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-520
Expression Tag6xHis
Purity>90%
AA SequenceMSDQNKTKDYSKYFDFSDARVVSEDDEKKIIKIKGREITIYDQPNYRAGRKRKREDVQVL RPENLIPEEVANLGAGKKFLIRTYGCQMNVHDSENMSGLLLGMGFEETTETEEADIILLN TCAIRENAENKVFGEIGNLKPLKLEKPELIIGVCGCMSQEESVVGKIMEKHQHIDLIFGT HNIHRLPHLLRDAIFGKEMVIEVWSKEGDIVENMPRSRKNKTQAWVNIMYGCDKFCTYCI VPYTRGKERSRLPEDIIAEVRDLARQGYKEITLLGQNVNAYGKDLPGSYRLGDLMNEIHK IDIPRVRFTTSHPRDFDDHLIEVLAQGGNLVEHIHLPVQHGNSDILKLMGRKYTREQYIT LANKIKQAIPNASFTTDLIVGFPNETEEQFQDMLSLVEEIQFDAAYTYIYSPREGTPAAR MEDNVPMSVKKERLARLNALVNDISNKRNLDYQDKIVEVLVEGESKKNTDVLAGRTRTNR LVNFVGPKSAIGEIVYVKITEAKTWSLDGEIVSAPEVKAQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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