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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP765306LEVYeast1mgQuote
EP765306LEVE. coli1mgQuote
BP765306LEVBaculovirus200ugQuote
MP765306LEVMammalian Cell200ugQuote

Protein Information

SpeciesLeptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
UniProt IDQ72PA4
Gene NamemiaB; Locus:LIC_12570
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMSVLEQEKKSGKVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRE NAHAKIYNRLQSLGYLKKRNPNLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPE LIQRIRKGENSISLTRLSKIETYDEIEPRVVNGIQAFVTIMRGCNNFCTFCVVPYTRGRE RSRDPKSIVREVQDLVQKGIRQITLLGQNVNSYKEQDTDFAGLIQMLLDETSIERIRFTS PHPKDFPTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVP DVGITTDIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEV KSARLTKLVDLQTSISHEQNRARIGRVYSILIENISRKSEKQLCGRTPCGRMTVFPLPQE TNVSGMIGSTVSVQIESATSATLKGRILA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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