Request QuoteCatalog Number: xP761842DDHSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP761842DDHYeast1mgQuote
EP761842DDHE. coli1mgQuote
BP761842DDHBaculovirus200ugQuote
MP761842DDHMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303)
UniProt IDQ72DE5
Gene NamemiaB; Locus:DVU_0984
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMHDRTFHIETFGCQMNVNDSDWLARALMERGFSPAPFGEARLTIVNTCSVRDKPEQKVYS LLGRIRQATSKKPDAFVAVGGCVAQQIGSGFFSRFPQVRLVFGTDGLAMAPQALDRLVEE PDLKLSLLDFSEDYPERDAVLGQGAVPASVFVNIMQGCDNFCAYCIVPYTRGRQKSRATG TILDECRALLDRGAREITLLGQNVNSFGQDSHGDGTTFAQLLHKVAALPGLERLRFVTPH PKDIAPEVVEAFGTLPNLCPRLHLPLQAGSDRILKLMGRRYDMARYLRIVDDLRAARPDI VLSSDIIVGFPGETEEDFMETMDALETVGYAASYSFCYSDRPGTRAEMLPDKLSREVKLE RLERLQTLQNRLTERCLQDMVGRKVEVLLEGMSRKPGDEGDSWQGRDPYGNLVNVALPQG SDVRGRFLPVVVAQAKKHSLLAEQAGAPW
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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