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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP712781Yeast1mgQuote
EP712781E. coli1mgQuote
BP712781Baculovirus200ugQuote
MP712781Mammalian Cell200ugQuote

Protein Information

SpeciesIdiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)
UniProt IDQ5QYC5
Gene NamemiaB; Locus:IL0941
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-479
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLYIKTWGCQMNEYDSTKMAELLHSTHGYDVAEEAEDADLILLNTCSIREKAQEKVF HQLGRWKNLKKNNPNLLIGVGGCVASQEGNEIRARAPFVDIIFGPQTLHRLPEMVNQVSE THAPMVDVSFPEIEKFDRLAEPKADGASAFVSIMEGCSKYCSFCVVPYTRGEEVSRPVDD VIYEIAQLAEQGVREVNLLGQNVNAYRGEHYDGEVCRFAELLHLVAAIDGIDRIRYTTSH PVEFTDDITEAYKTIPELVSHLHLPVQSGSDRILTMMKRGHTALEYKSKIRALKKARPDI AMSSDFIIGFPGESDADFEATMDLIQSIDFDMSFSFIYSARPGTPAADLPDDISEETKKK RLQLLQQRLNQQSMAHARRMLETEQRILVTGPSKKNPMELTGRTENNRVVNFVGQPHMIG QFVDVRITEVLPNSLRGELIREEADMGLRVDTAPEIILQRGKSSEPDELGVVRMPRQAS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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