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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP689399Yeast1mgQuote
EP689399E. coli1mgQuote
BP689399Baculovirus200ugQuote
MP689399Mammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus kaustophilus (strain HTA426)
UniProt IDQ5L0E8
Gene NamemiaB; Locus:GK1303
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-531
Expression Tag6xHis
Purity>90%
AA SequenceMNEKQRLEQTGQIKTESHPADRKSALDRLKEKTTKDYEKYFTSVFLPPNLKEAKKRGKEE VKYVKDFSIPEEFRGMGRGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPEEANVI LLNTCAIRENAENKVFGELGYLKPLKTTNPDLLLGVCGCMSQEESVVKKILKQYQYVDLI FGTHNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGKIKAWVNIMYGCDKFCT YCIVPYTRGKERSRRPEDIIQEVRQLAAQGYKEITLLGQNVNAYGKDFTDIQYGLGDLMD ELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYTRE EYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGT PAANMKDNVPMEVKKERLKRLNDLVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAGYT RKNKLVHFVGPKSLIGQLVNVRITQAKTWTLTGELVNEAIEVNEHGKIYAG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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