Request QuoteCatalog Number: xP758620GBKSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP758620GBKYeast1mgQuote
EP758620GBKE. coli1mgQuote
BP758620GBKBaculovirus200ugQuote
MP758620GBKMammalian Cell200ugQuote

Protein Information

SpeciesGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
UniProt IDQ74B44
Gene NamemiaB; Locus:GSU2198
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMTENKYLYVETFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKV YGHLGRFKGVRSRKKGFLLGVGGCVAQQEGERLLQKVPWLDLVFGTHNLHLLPEIVRAAE RGERRAEVGFIDNETRLDLFPETGGEGGVTRFVTVMQGCDNFCSYCIVPYVRGREISRRS SDIIDEVRKSVAEGVKEVTLLGQNVNSYGLKTEGELSFAGLIRRIAEVEGLERIRFTTSH PKDISPELIACFAEVPKLCGHIHLPAQSGSDAVLARMNRGYTRAGYLEKVEALRAARPGI QFTGDMIVGFPGETEEDFQATISLMEEVRYADLFSFIYSPRPETAAAGIRDTVSRKEKQS RLDRLQTLQQQMKRERNISFVGTRQLVLVEGVSKRGDQLYGRIDGNRIVNFAADQSLIGT MAEVTITQDYQNSLLGEIVTDKGDAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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