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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP754971MLAYeast1mgQuote
EP754971MLAE. coli1mgQuote
BP754971MLABaculovirus200ugQuote
MP754971MLAMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium paratuberculosis
UniProt IDQ73W15
Gene NamemiaB; Locus:MAP_2846c
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-517
Expression Tag6xHis
Purity>90%
AA SequenceMTCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRA AEGAEVADVVVFNTCAVRENADNKLYGNLSHLAPRKRGNPEMQIAVGGCLAQKDREAVLR RAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQFPSSLPSARESAYAAWVSIS VGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGS DRVLRAMRRSYRAERFLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARF AAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQESISLQGNQALVGQTVELLVA TGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGVLSHRR TRAGDAHAAGRRPRGVGLGMPAVGPPAGPAQPVGCAS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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