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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP702649Yeast1mgQuote
EP702649E. coli1mgQuote
BP702649Baculovirus200ugQuote
MP702649Mammalian Cell200ugQuote

Protein Information

SpeciesSilicibacter pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
UniProt IDQ5LLM0
Gene NamemiaB; Locus:SPOA0002
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-439
Expression Tag6xHis
Purity>90%
AA SequenceMSDPKKLFIKTYGCQMNVYDSERMAEALGGQGYVETQSAEDADMILLNTCHIREKAAEKV YSELGRFKGLKAAKPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQSYHRLPELEAKTR AGEKALDTDFPEEDKFEKLKGRPKAKRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRPA DRILREANELVERGVREITLLGQNVNAYHGAGPNGDMTLAGLIWELDKIDGLERIRFTTS HPNDMADDLIEAHGTCAKLMPYLHLPVQAGSDKILKRMNRSHTAESYLRLIERIRAARPD IVMSGDFIVGFPEETEADFQDTLDLVEQVRYGYAYSFKYSTRPGTPAAERAQVDPAEADD RLQRLQAVITRHQREIQDGMVGREVSVLFEKPGRQPGQMVGKSEYLHAVHVQDPGLEAGQ IARVRIVASGANSLAGELA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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