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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP683502Yeast1mgQuote
EP683502E. coli1mgQuote
BP683502Baculovirus200ugQuote
MP683502Mammalian Cell200ugQuote

Protein Information

SpeciesBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
UniProt IDQ5LJ70
Gene NamemiaB; Locus:BF0027
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMNELTGADFKSATADDNKKLFIETYGCQMNVADSEVIASVMQMAGYSVAETLEEADAVFM NTCSIRDNAEQKILNRLEFFHSMKKKKKHLIVGVLGCMAERVKDDLIEHHHVDLVVGPDA YLTLPELIASVEAGEKAMNVELSTTETYRDVIPSRICGNHISGFVSIMRGCNNFCTYCIV PYTRGRERSRDVESILNEVADLVSKGYKEITLLGQNVNSYRFEKEGGEVVTFPMLLRLVA EAAPGIRVRFTTSHPKDMSDETLEVIAQVPNVCKHIHLPVQSGSSRILKLMNRKYTREWY LDRVAAIKRIVPDCGLTTDIFSGFHSETEEDHRESLSLMEACGYDAAFMFKYSERPGTYA SKHLEDNVPEEIKVRRLNEIIALQNRLSAESNNRCIGKTYEVLVEGVSKRSRDQLFGRTE QNRVVVFDRGTHRIGDFVNVRITEASSATLKGEEVFS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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