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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP728023Yeast1mgQuote
EP728023E. coli1mgQuote
BP728023Baculovirus200ugQuote
MP728023Mammalian Cell200ugQuote

Protein Information

SpeciesLeifsonia xyli subsp. xyli (strain CTCB07)
UniProt IDQ6AE03
Gene NamemiaB; Locus:Lxx15990
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-535
Expression Tag6xHis
Purity>90%
AA SequenceMSIIDHHSVVPRSEAAVGEDGRARTYEVRTFGCQMNVHDSERLSGSLEAAGYVPANGEEA DIVVINTCAVRENADNKLYGNLGHLASVKRRHAGMQIAVGGCLAQKDKNVILEKAPWVDV VFGTHNMGALPRLLERARHNDAAEIEILEALETFPSTLPTKRDSSFSGWVSISVGCNNTC TFCIVPALRGKEKDRRPGDILAEVQALVDEGAVEVTLLGQNVNSYGVEFGDRQAFSKLLR AAGQIEGLERIRFTSPHPAAFTEDVIDAMAETPNVMPQLHMPLQSGSDRILRSMRRSYRS EKFLGILDRVRAKLPGAAISTDIIVGFPGETEEDFLDTLRVVEAARFASAFTFQYSIRPG TPAATMAGQVPKEVVQERYDRLIALQERISLEENEKLIGRDVELLVATGEGRKDADTRRL SGRARDSRLVHFELPAGSEVPRPGDVALVRVTQAAPHYLIADAAGGPLRVRRTRAGDAWD RAEAESCAAPSLSGGRAAAAVGRVSLGLPTLRTREPLTSPGVGTMPLYDPTDGQR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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