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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP679195Yeast1mgQuote
EP679195E. coli1mgQuote
BP679195Baculovirus200ugQuote
MP679195Mammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
UniProt IDQ4K5I3
Gene NamemiaB; Locus:PFL_5432
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY SQLGRWRELKLANPDMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDVARI TKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD VIAEIIHLAENGVREVTLLGQNVNGYRGQTHDGRMADLAELIRVVAAIDGIERIRYTTSH PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGI CISSDFIVGFPGETEKDFQQTMKLIEDVGFDFSYSFVYSQRPGTPAADLADETPEAVKKE RLNALQHRLNQQGFEISRQMVGSTQRILVTDYSKKDPGELQGRTENNRIVNFRCDNPTLI GQFADVYIDSAQPHSLRGSLLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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