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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP678233Yeast1mgQuote
EP678233E. coli1mgQuote
BP678233Baculovirus200ugQuote
MP678233Mammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium jeikeium (strain K411)
UniProt IDQ4JV79
Gene NamemiaB; Locus:jk1114
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMTQTIQTQTGKNSGAGRTYEVRTFGCQMNVHDSERLSGLLEDSGYVPASEGVTPDVVVFN TCAVRENADKRLYGTLGQMKAIKDEHPGMQIAVGGCMAQKDKQTVVDKAPWVDVVFGTHN LGSLPTLLERSAHNHKAEVEIFDALEEFPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVP SLRGKEQDRRPGEILAEVKALVEQGVSEVTLLGQNVNAYGVNFADETLERDRTAFSKLLR ACGEIEGLERLRFTSPHPAEFTDDVIDAMAETPAVCPQLHMPLQSGSDKVLKDMKRSYRS KKFLAILDKVRERIPHAAITTDIIVGFPGETEEDFQATLDVVEKARFSSAFTFQYSPRPG TPAAEMENQVPKAVVQDRYERLLALQERISEEENRKLIGTTQELLVQADGRKNDRTQRRS GRSRDGRLVHFTPEGDVRAGDVVEVVITDAAPHFLIADGGVASHRRTRAGDMTELGETPT TAPVGVGLGMPSIKKPEPTTAGGCSTGGCGCE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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