Request QuoteCatalog Number: xP669861MVNSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP669861MVNYeast1mgQuote
EP669861MVNE. coli1mgQuote
BP669861MVNBaculovirus200ugQuote
MP669861MVNMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium leprae
UniProt IDQ49842
Gene NamemiaB; Locus:ML0989; ORFs:B2235_C2_195
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-517
Expression Tag6xHis
Purity>90%
AA SequenceMVTRDAGMADDAATGSTRTYQVRTYGCQMNVHDSERLAGLLEAAGYQRAADEADVGDADV VVFNTCAVRENADNRLYGNLSHLAPRKRNNPDMQIAVGGCLAQKDKHTVLSKAPWVDIVF GTHNLGSLPTLLDRARHNKVAQVEIVEALQHFPSSLPSARESDYAAWVSISVGCNNSCTF CIVPSLRGKEVDRSPADILAEVEALVADGVLEVTLLGQNVNAYGVSFADPALARNRGSFA ELLRSCGSIDGLERVRFTSPHPAEFTYDVIEAMAQTPNVCPSLHMPLQSGSDRVLRAMRR SYRVERYLGIIGQVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRQVRFTAAFTFQYS KRPGTPAAELGGQLPKAVVQERYERLVELQEQICMEENRVLIGRIVELLVTTGEGRKDAR TARRTGRARDGRLVHFALDTPWEAQVRPGDIITVMVTEAAPHHLIADAGILTHRRTRAGD AHAARQCPRSIGLGQIPLGMPSVGQHSASIGSAGCAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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