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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP658671GBJYeast1mgQuote
EP658671GBJE. coli1mgQuote
BP658671GBJBaculovirus200ugQuote
MP658671GBJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
UniProt IDQ39TA3
Gene NamemiaB; Locus:Gmet_2296
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-441
Expression Tag6xHis
Purity>90%
AA SequenceMTGEKLLYIETFGCQMNVSDSEKVASLLRGEGYSQTPDSSEADLIIVNTCSVRAKAEHKV YSYLGRFRKLKRDRRLLLGVGGCVAQQEGERLLKRVPWLDLVFGTHNLHLLPEMVRAAEQ GERRAAVDFIDNEARLDLFPQADEGGGVTRFVTVMQGCDNFCSYCIVPYVRGREISRRSV EIIGEIRSAVAGGVREVTLLGQNVNSYGLKTPGELSFAGLLREISAIDGLERIRFTTSHP KDISPELIACFAELPKLCGHIHLPAQAGSDSILARMNRGYTRQEYLEKVAALRAARPEIL ITGDIIVGFPGETEADFLQTLSLMEEVRYTDIFSFAYSPRPETAAASLGDRIMRKETTER LERVQGLQRDMTIERHAGFVGTCQAVLVEGMSKRGDQLYGRTDGNLIVNFAGNPSLAGSL VDVRITRGYPNSLLGELAVFQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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