Request QuoteCatalog Number: xP710182BWSSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP710182BWSYeast1mgQuote
EP710182BWSE. coli1mgQuote
BP710182BWSBaculovirus200ugQuote
MP710182BWSMammalian Cell200ugQuote

Protein Information

SpeciesBrucella abortus biovar 1 (strain 9-941)
UniProt IDQ57AB1
Gene NamemiaB; Locus:BruAb1_2127
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDA DLVLLNTCHIREKASEKLYSALGRLRKMRDARAADGKELTIGVAGCVAQAEGQEILRRAP NVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHLPAPRREETRKRGVSAFLTV QEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG REWGLGELLFRLARIPGIARLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRI LKAMNRRHKADEYLRLIERIRNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQA YSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQYAFQDSMIGRKMDVLLEKPG REAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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