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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP658932Yeast1mgQuote
EP658932E. coli1mgQuote
BP658932Baculovirus200ugQuote
MP658932Mammalian Cell200ugQuote

Protein Information

SpeciesPelodictyon luteolum (strain DSM 273) (Chlorobium luteolum (strain DSM 273) )
UniProt IDQ3B6A6
Gene NamemiaB; Locus:Plut_0237
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMPEGRRFYIQTFGCQMNEADSGIIARVLLDAGFRRADTEQDADVVLLNTCAVRENAVEKI AHLLEHLKGAKKRRKTLQVGVLGCVPQHQREEMFSRFPAIDFIAGPDSYRRLPSLIDDAA SAVRSAMLDFDPSETYVGIRQVREGRISAFIPVMRGCNNMCAFCVVPFTRGRERSQPLAM VTGEARELAEAGYREITLLGQNVNSYSDPASGATFSALLDAVALAAPDVRIRFTTSHPKD ISVGLIDTIARRPNICRHVHLPVQSGSDSVLRRMNRGHGISEYLEKIRIIRDALPGVTLS TDIIAGFCGEREEDHRATLDLLRTVRFDAAFMFHYSTREGTLAARTLPDDVAETDKKRRL QEIIDLQQEISAENNRRQVGTVAEVLAESESRRSPGRLLGRTDGNRAVVFDRGECMPGDL VRVRLTSSTSATLSGSREGLIRAFLS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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