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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP672269Yeast1mgQuote
EP672269E. coli1mgQuote
BP672269Baculovirus200ugQuote
MP672269Mammalian Cell200ugQuote

Protein Information

SpeciesPelagibacter ubique (strain HTCC1062)
UniProt IDQ4FNN5
Gene NamemiaB; Locus:SAR11_0383
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMLKKIFIKTFGCQMNEYDSNRIFDTVKKIGFEKTEKYEDANCYLLNTCHIRDKAKEKVYH EIGRVKKIFREKKKPIVVVAGCVAQAENQEMLKREPYIDIVIGPQSYHKINEAILNHLKN KKKEEETEFDTISKFNYLSQIKNKDSKVSSFLTIQEGCDKFCHFCVVPYTRGPEYSRPFD QIINEAKELVQSGAKEIILLGQNVNAYSYDEEGKKYRLSDLLIKLDSFDKLERIRYTTSH PKDMTDDLINVYKTSSKLMPLVHLPVQSGSNKILNLMNRKHTIEEYLLVYEKLRKINPKI EFSSDFIIGYPEEDEQDFKMTMELIEKVKFINSYSFIFSPRPGTVAANLTLVDQKKSKQR LEIIQEKLFNNQIKKNKSLENKILNVLVENKMKDGIKLFGRTEYMTSVIFDGNIENIGKL VQVEIISSNQNSLFGKLTESSKKKVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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