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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP650790Yeast1mgQuote
EP650790E. coli1mgQuote
BP650790Baculovirus200ugQuote
MP650790Mammalian Cell200ugQuote

Protein Information

SpeciesXanthomonas oryzae pv. oryzae (strain MAFF 311018)
UniProt IDQ2P266
Gene NamemiaB; Locus:XOO2606
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-484
Expression Tag6xHis
Purity>90%
AA SequenceMPGTSVSDLSTATAVDAPALLPLPVARPSAPAVVRGKLYIKTHGCQMNEYDSAKMADVLA ASEGLELTDNPEEADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQ EGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRAEGP SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYL HLPVQAGSDRILSAMKRGYTALEFKSRIRKLRAVRPDISISSDFIVGFPGETEADFEKTM KLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPETVKQARLARLQAHISAHAASISQSMVG SVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD DSAH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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