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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP669182Yeast1mgQuote
EP669182E. coli1mgQuote
BP669182Baculovirus200ugQuote
MP669182Mammalian Cell200ugQuote

Protein Information

SpeciesDehalococcoides sp. (strain CBDB1)
UniProt IDQ3ZYS0
Gene NamemiaB; Locus:cbdbA1340
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-418
Expression Tag6xHis
Purity>90%
AA SequenceMPGYYLWTIGCQMNQAESDRLGRLFELWGYSLADKAEDAELVLVNSCVVREHAENKVVNR LHLLRSLKNKNPKLKIALTGCLVGQDISLIKKKFPFVDYIFGPGSMPDWREIPEGFILPL RPPVSANVTIMQGCNNFCTYCVVPYRRGREKSRSIAEIGCEVAELVRRGSREVVLLGQNV DSYGHDLPEKPCLADLLSALHDITGLLRIRFLTSHPKDISQKLIDAMAHLPKVCRSLSLP VQSGDDTILASMRRGYTNQQYRELVERIKTAMPDISLQTDLIVGFPSENEEQFNQSYKLM ADIGYDAIHVAAYSPRPQTVAARDMADDVPVIEKKRRLKLIEDLQKETVGKANAALMDTF AEVLVEGLQKNKWQGRTLGGKLVFLESDLPLEGCLVKVKIFKTSPWSLQAKLVNILES
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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