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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP674028Yeast1mgQuote
EP674028E. coli1mgQuote
BP674028Baculovirus200ugQuote
MP674028Mammalian Cell200ugQuote

Protein Information

SpeciesDechloromonas aromatica (strain RCB)
UniProt IDQ47A75
Gene NamemiaB; Locus:Daro_3527
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMPKKLFIRTFGCQMNEYDSDKMADVLNASEGVIKTDNPEEADIILFNTCSVREKAQEKVF HDLGRVRHLKKLNPNLVIGVGGCVASQEGDAIIARAPYVDVVFGPQTLHRLPQLIAERKA KGKAAVDVSFPEIEKFDAMPPSEIKGASAFVSIMEGCSKFCTFCIVPYTRGGEVSRPFND VLTEVADLAAHGVGEVTLLGQNVNAYRGDMEGTEEKADLALLIEYIAEVPGIERIRYTTS HPREMTQSLFDTYVKVPKLVSHLHLPVQAGSDRVLAAMKRGYTTLEYKSIIRKLRAARPD ISLSSDFIVGFPGETDEDFEKTMKLIDDVGFDSSFSFIYSPRPGTPALELDDSTPADVKS ARLLRLQKRIDEQAQAISQTMVGSIQRVLVEGTSKKDVHELAGRTDNNRIVNFSGNPRLI NTFVDVRITSSLSHTLRGEIVIRED
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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