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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP672799Yeast1mgQuote
EP672799E. coli1mgQuote
BP672799Baculovirus200ugQuote
MP672799Mammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain NATL2A)
UniProt IDQ46JG6
Gene NamemiaB; Locus:PMN2A_0871
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-463
Expression Tag6xHis
Purity>90%
AA SequenceMTTVQLQKTNQPSIKASRGSYWITTFGCQMNKADSERMSGILEYMGYYPAEEELKADLVL YNTCTIRDSAEQKVYSYLGRQAIRKRSLPNLKIVVAGCLAQQEGESLLRRVPEIDLLMGP QHCNRLESLLNQVDSGQQVLATEEQFILEDITTPRRDSSICGWVNIIYGCNERCTYCVVP SVRGKEQSRTPEAIKSEVEDLAKSGYKEITLLGQNIDAYGRDFQSQNKEDSAQVTLSYLL KYIHDIEGIERIRFATSHPRYFTKELIDTCSELPKVCEHFHIPFQSGSNKILKNMGRGYT IESYKNIINYIKAKIPKAAISGDAIVAFPGESETDYEQTLSLIDEIKFDHVNTAAYSPRP NTPAATWPRQLNEDIKVKRLREINSLVENIAKERNQRYKNTSQEILIENINPKDSFQLMG RTRTNRLTFFPRSLKNGVENKLGELIKVKITDVRPFSLTAKLL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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