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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP667093Yeast1mgQuote
EP667093E. coli1mgQuote
BP667093Baculovirus200ugQuote
MP667093Mammalian Cell200ugQuote

Protein Information

SpeciesNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
UniProt IDQ3JEH9
Gene NamemiaB; Locus:Noc_0237
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMAKKLYIKTHGCQMNEYDSSRMADVLRESHGLELTSDPEQAEVLLLNTCSVREKAQEKVF SQLGRWRQLKQSRPELVIGVGGCVASQEGETIRVRAPYVDLVFGPQTIHRLPAMLTQAKA SSQPVVDLSFPEIEKFDKLPEPRAEGPTAFVSVMEGCSKYCSFCVVPYTRGEEISRPFDD VIAEIVGLAEQGVREITLLGQNVNAYRGSIGEGDTADLALLIHYVASIEGIERIRFTTSH PVEFSDSLIQAFAEVPALVSHLHLPVQSGSDRMLSLMKRGHTVLEYKAKLRKLRQVRPDI SISSDFIVGLPGETEADFQATLALVDEVGFDHSFSFIYSPRPGTPAASLPDCTPIALKKE RLAILQGRLRALEIAISRGMVDTVQQVLVERPSKKDPTMLAGRTANNRVVNFSASQDMVG QFVNIRITEALPNSLRGIVLDAKRRCA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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