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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP677561Yeast1mgQuote
EP677561E. coli1mgQuote
BP677561Baculovirus200ugQuote
MP677561Mammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
UniProt IDQ48DN8
Gene NamemiaB; Locus:PSPPH_4387
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY SQLGRWRELKLANPEMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARI TRLPQVDVSFPEIEKFDHLPEPRVDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD VLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTTSH PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVPGI SISSDFIVGFPGETEKDFDNTMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALKKE RLAALQHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQGRTENNRIVNFRCNNPKLI GQFADVHIDDAQPHSLRGSLLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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