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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP677491Yeast1mgQuote
EP677491E. coli1mgQuote
BP677491Baculovirus200ugQuote
MP677491Mammalian Cell200ugQuote

Protein Information

SpeciesColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
UniProt IDQ47Y80
Gene NamemiaB; Locus:CPS_3572
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-480
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLYIKTWGCQMNEYDSQKMAELLDSTHGFSLVEEAEQADVILLNTCSIREKAQEKVF HQLGRWKNLKDKKPDLLIGVGGCVASQEGDSIRKRAPFVDMIFGPQTLHRLPEMLNQLQH SKSPIIDVSFPEIEKFDRLPEPKADGASAFVSIMEGCSKYCTFCVVPYTRGEEVSRPLDD VLYEIAQLAEQGVREVNLLGQNVNAYRGETHDGSICRFADLVRLVATIDGIDRIRYTTSH PVEFTDDIIEAYTDVPELVNHLHLPVQSGCDRILTQMKRGHTALEYKSQIRKLKKVRPEL SMSSDFIIGFPGETDEDFTATMDLIKAVDFDLSFSFIYSARPGTPAADLPDDISDQTKKD RLKLLQEQITHQALRIARQMLNTEQRVLVEGPSRKNPMELRGKTENNRTVNFVAPHSVIG QFVDIKITDVVANSLRGELVREEKEMGLRIAHSPADILANNHHMATPSNLDELGVGTFTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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