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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP690396EZXYeast1mgQuote
EP690396EZXE. coli1mgQuote
BP690396EZXBaculovirus200ugQuote
MP690396EZXMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
UniProt IDQ51470
Gene NamemiaB; Locus:PA3980
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMAKKLFIETHGCQMNEYDSSRMADLLGEHQALEVTENAAEADVILLNTCSIREKAQEKVF SKLGMWRELKQQNPDLVIGVGGCVASQEGAAIRERAPYVDVVFGPQTLHRLPEMIDAARS TRKPQVDVSFPEIEKFDRLPEPRVDGPTAFVSVMEGCSKYCSFCVVPYTRGEEVSRPFDD VIAEVIHLAENGVREVTLLGQNVNGFRGLTHDGRLADFAELLRVVAAVDGIERIRYTTSH PLEFSDALIQAHAEVPELVKFIHLPVQSGSDRVLAAMKRNHTVLEYKSRIRKLKAAVPDI CISSDFIVGFPGETEKDFEQTMKLVEDVGFDFSFSFIYSARPGTPAADLADDLPEEVKKQ RLQILQSRIHQQGYEISRRMVGSTQRILVTDFSKKDPGMLQGRTENNRIVNFRCDNPRLI GQFAQVHIDDALPHSLRGTLIDSTLH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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