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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP701140HTAYeast1mgQuote
EP701140HTAE. coli1mgQuote
BP701140HTABaculovirus200ugQuote
MP701140HTAMammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
UniProt IDQ57163
Gene NamemiaB; Locus:HI_0019
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVF HQLGRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG GKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDD VLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH PIEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDI QISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQ RLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMIG KFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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