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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP608160RLOYeast1mgQuote
EP608160RLOE. coli1mgQuote
BP608160RLOBaculovirus200ugQuote
MP608160RLOMammalian Cell200ugQuote

Protein Information

SpeciesRhodococcus sp. (strain RHA1)
UniProt IDQ0S1P3
Gene NamemiaB; Locus:RHA1_ro06771
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-505
Expression Tag6xHis
Purity>90%
AA SequenceMDTIDQTPHRSYEVRTYGCQMNVHDSERLSGLLEDAGYTKAAAGQAPDLVVFNTCAVREN ADNKLYGNLSHLAPAKERNPDMQIAVGGCLAQKDRDVVVKKAPWVDVVFGTHNIGSLPAL LDRARHNQRAEVEILEALEAFPSTLPAKRESAYAGWVSISVGCNNTCTFCIVPALRGKEV DRRPGDILAEVQALVNEGVVEVTLLGQNVNAYGVSFADPDQPRDRGAFAALLRACGQIDG LERVRFTSPHPAEFTDDVIEAMAETPNVCPQLHMPLQSGSDRVLKAMRRSYRKSRFLGII DKVRTAMPHAAITTDIIVGFPGETEEDFQDTLDVVRQARFTSAYTFQYSKRPGTPAAEMD EQLPKAVVQERYERLIALQEQITLEENQKLVGAEVELLVAAGEGRKNAETARMSGRARDG RLVHFRPEGNLDGTVRPGDVVTVVVSAAAPHHLVADTPVLTHRRTRAGDSFEKGVTPKTP PIGVGLGLPQIGAPAPLPVQMGCNA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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