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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP625123Yeast1mgQuote
EP625123E. coli1mgQuote
BP625123Baculovirus200ugQuote
MP625123Mammalian Cell200ugQuote

Protein Information

SpeciesHelicobacter pylori (strain HPAG1)
UniProt IDQ1CUN4
Gene NamemiaB; Locus:HPAG1_0271
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-437
Expression Tag6xHis
Purity>90%
AA SequenceMKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEI GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAI DYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILKEAEKLA NNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDGF LERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGISTDIIV GFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSYSRLERLQNR HKEILEEKAKLEVGKTHVVLVENRREMDNQIVGFEGRSDTGKFIEVTCKEKRNPGELVKV EIISHSKGRLIAAIKGN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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