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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP601818Yeast1mgQuote
EP601818E. coli1mgQuote
BP601818Baculovirus200ugQuote
MP601818Mammalian Cell200ugQuote

Protein Information

SpeciesMaricaulis maris (strain MCS10)
UniProt IDQ0AK79
Gene NamemiaB; Locus:Mmar10_3033
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMSTTRKRLFIKTYGCQMNVYDSDRMKDVLTPLGYESAETPEGADLVILNTCHIREKAAEK VYSELGRLRPLKDEKAASGGMTIAVAGCVAQAEGAEIMKRAPVVDLVVGPQTYHKLPELI AQAHRAKGEALDTEFEVEDKFDRLGSDRQVEGYSAFVTVQEGCDKFCTFCVVPYTRGAEW SRPVDQIVAEIRALAAKGIREVTLLGQNVNAFHGAPPSGREAEGAWGLGQLVRHVALIGG IERIRFTTSHPRDMDEVLIQAFADTPKLMPYFHLPVQAGSDKILKSMNRQHTAEEYVAII DRLRAARPDIAISGDMIVGFPGETDADFEATLDLVRRVKFASCFSFKYSKRPGTPGAAMF NQVDEGVKSARLAVLQELLSDQQAAFNESMIGRTLPVLFEKPGRMGGQLHGRSPYLQSVH VDGPAELIGQVGEVRIEAASRNSLSGSLTGSKRSAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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