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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP601787Yeast1mgQuote
EP601787E. coli1mgQuote
BP601787Baculovirus200ugQuote
MP601787Mammalian Cell200ugQuote

Protein Information

SpeciesNitrosomonas eutropha (strain C91)
UniProt IDQ0AF59
Gene NamemiaB; Locus:Neut_1789
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-443
Expression Tag6xHis
Purity>90%
AA SequenceMSSKLYIKTFGCQMNEYDSAKMADILLSEKNMELTEVPEEADLILFNTCSVREKAQEKVF HDLGRVRHLKNSKPDLLIGVGGCVASQEGSEIVRRAPFVDLVFGPQTLHRLPELIDARRR TGQSQVDITFPEIEKFDRLPPARTTGATAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD VLTEVAGLVIQGVKEVTLLGQNVNAYYDKTSGEGDIDFATLLDYIHEIPGLVRIRYTTSH PREFTARLIETYQRLPKLVGHVHLPIQSGSDRILAAMKRGYTIIEYKSIIRKLRTIRPNI SISSDFIVGFPGETDTDFEETMKLIDDVKFDESFSFIYSPRPGTPASDLPDDTPYRIKLA RLHQLQEKIQRNAQMISQSMVDTIQRVLVEGPSKKDPNEFCGRTDNNRVVNFAGHAGLVG SFVDIKITAVSSHTLRGEISDMQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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