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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP602368Yeast1mgQuote
EP602368E. coli1mgQuote
BP602368Baculovirus200ugQuote
MP602368Mammalian Cell200ugQuote

Protein Information

SpeciesAlkalilimnicola ehrlichei (strain MLHE-1)
UniProt IDQ0ABN9
Gene NamemiaB; Locus:Mlg_0394
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-448
Expression Tag6xHis
Purity>90%
AA SequenceMTGRVYVKTHGCQMNEYDSDKMADVLVKERGYTRVDSPGDADVILLNTCSVREKAQEKVF SELGRWKDYKTRNGAVIGVGGCVASQEGEAIVQRAPHVDVVFGPQTLHRLPEMIDRARDG GRSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEISRPFEDV IAEVASLAEQGVREVTLLGQNVNAYRGPMADGTVCDLALLIHYVAALDGIGRIRFTTSHP VEFSDSLIEAYREEPKLAGHLHLPVQSGSDLVLKLMKRGHTAAEYLDKIERIKAARPGIS IASDFIVGYPGESEADFEDTLRLIEAVGFDQSFSFLYSPRPGTPAASLSDSTPAEVKRER LYRLQETINANARRISESMVGTVQRVLVDGRSRKDPNEISGRTENNRVVNFAGHPRLIGH FVEVRITEAKPNSLRGELLGLDDDMAAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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