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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP629985Yeast1mgQuote
EP629985E. coli1mgQuote
BP629985Baculovirus200ugQuote
MP629985Mammalian Cell200ugQuote

Protein Information

SpeciesNitrobacter hamburgensis (strain X14 / DSM 10229)
UniProt IDQ1QS74
Gene NamemiaB; Locus:Nham_0021
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMTPPRKLHIKSYGCQMNVYDAQRMVDTLAAEGFVETASAEEADLVILNTCHIREKASEKV YSELGRLRAAKDQAARDGREMSVVVAGCVAQAEGDEIIRRAPVVDVVVGPQSYHHLPQLL ARAKADGRALETEFPVEDKFGFLPQPSRQAIRARGISAFVTVQEGCDKFCTFCVVPYTRG AEVSRPVAKIVEDVQRLADNGVREITLIGQNVNAYHGDGPDGRPWPFGKLLHRLADIPGI VRLRYSTSHPRDVEDSLIEAHRDLGALMPFVHLPVQSGSDRILEAMNRRHTADDYRRVVD RFRHVRQDIAFSSDFIVGFPGETEQDFTATLALVTQIGYAGAYSFKYSPRPGTPAAEMKE TVSTADMDERLVRLQNLIDSQQSAFNRAAVGTTVDVLFERAARNPGQIVGRTAYLQPAHV VASADIIGQILPVTVASLERYSLLGSLASAPASRASADDAPPVGASSPAIMGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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