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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP612625DZQYeast1mgQuote
EP612625DZQE. coli1mgQuote
BP612625DZQBaculovirus200ugQuote
MP612625DZQMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
UniProt IDQ0KE93
Gene NamemiaB; Locus:H16_A0528
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEEADVILFNTCSVREKAQEKVFS ELGRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRT GQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV LAEVAGLAEQGVREVTLLGQNVNAYRGKMGDTTEIADFALLIEYVAEIPGIERIRYTTSH PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRILMAMKRGYSVLEYKSIIRKLRVLRPDM SMSSDFIVGFPGETDADFEKLMAMIEEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR RLQRLQATIEENVQRISQNMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQA GRDRLVGQLVDVSITQAFPHSLRGEIVVRQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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